Rodent Sperm Analysis (RSA) is a developed method that was designed to serve as an adjunct to the conventional desktop-only ecological risk assessment (ERA) process, as it is applied to mammals at contaminated terrestrial sites. While consistent with the ERA process, RSA advances mammalian assessment by recognizing that virtually all sites have been contaminated so long as to make the "risk" question no longer relevant; mammals have either succumbed from their chemical exposures or they are today, none-the-worse for living amidst contamination. RSA is the only method of its kind in the ERA arena - a direct health status assessment scheme evaluating the very mammals that occupy contaminated sites. For the Army-developed, formerly U.S. Government-patented, and ASTM International-certified (E3155-19)RSA method, Technical Guide 330 (TG330) provides RSA's underlying theory, a detailed method application description, and cautious instruction with regard to the interpretation of RSA application outcomes.